https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:45189 In situ hybridisation revealed that polyI:C offspring had: (1) SST mRNA reductions in the cingulate cortex and nucleus accumbens shell, regardless of MIA timing; (2) SSTR2 mRNA reductions in the cortex and striatum of GD19, but not GD10, MIA; (3) no alterations in cortical or striatal GAD1 mRNA of polyI:C offspring, but an expected reduction of PVALB mRNA in the infralimbic cortex, and; (4) no alterations in inhibitory markers in hippocampus. Maternal IL-6 response negatively correlated with adult offspring SST mRNA in cortex and striatum, but not hippocampus. These results show lasting inhibitory-related deficits in cortex and striatum in adult offspring from MIA. SST downregulation in specific cortical and striatal subregions, with additional deficits in somatostatin-related signalling through SSTR2, may contribute to some of the adult behavioural changes resulting from MIA and its timing.]]> Wed 26 Oct 2022 14:26:13 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:52465 Wed 11 Oct 2023 20:36:57 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:16819 Wed 11 Apr 2018 17:19:46 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:17021 Wed 11 Apr 2018 17:14:33 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:9724 Wed 11 Apr 2018 16:32:53 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:15023 Wed 11 Apr 2018 16:31:59 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:15130 Wed 11 Apr 2018 16:03:23 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:19642 Wed 11 Apr 2018 14:59:48 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:14888 Wed 11 Apr 2018 14:03:26 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:27445 Wed 11 Apr 2018 12:10:23 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:14891 Wed 11 Apr 2018 11:35:05 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:17101 Wed 11 Apr 2018 09:35:27 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:33905 Tnfa, mitogen-activated protein kinase 8 (Mapk8/Jnk1), and growth differentiation factor 9 (Gdf9) (P < 0.05). Mass spectrometry and cell signaling pathway analysis indicated upregulation of cellular pathways associated with acute phase signaling, and cellular survival and assembly. Apoptosis assessed by terminal deoxynucleotidyl transferase dUTP nick end labeling indicated significantly increased positive staining in the ovaries of LPS-treated neonates. These findings suggest that increased proinflammatory signaling within the neonatal ovary may be responsible for the LPS-induced depletion of the primordial follicle pool. These findings also have implications for female reproductive health, as the ovarian reserve is a major determinate of female reproductive longevity.]]> Wed 09 Feb 2022 15:58:52 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30359 Wed 04 Sep 2019 09:47:44 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:16906 A agonist muscimol or saline into the amygdala bilaterally and were subjected to a respiratory recording using whole body plethysmography. Presentation of acoustic stimuli (500-ms white noise, 40–90 dB) caused transient responses in respiratory rate that were proportional to the stimulus intensity, ranging from +13 ± 9 cpm to +276 ± 67 cpm for 40- and 90-dB stimuli, respectively. Inhibition of the amygdala significantly suppressed respiratory rate responses to the high-intensity stimuli (70–90dB). Submitting rats to the restraint stress significantly elevated the mean respiratory rate (+72 ± 8 cpm) and the dominant respiratory rate (+1 ± 12 cpm), as well as the fraction of high-frequency respiratory rate (+10 ± 3%). Inhibition of the amygdala by muscimol significantly suppressed these responses. We conclude that the amygdala is one of the key structures that are essential for expression of respiratory responses to stressful or alerting stimuli in rats.]]> Tue 31 Jul 2018 15:18:15 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:48670 Tue 28 Mar 2023 10:15:40 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:32927 Tue 14 Aug 2018 14:59:36 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:33669 Iba1, Gfap, IL1-β and TNF-α mRNA levels in the cingulate cortex (CC) in adult offspring exposed to maternal immune activation. Prenatal exposure to immune activation had a significant main effect on microglial IBA1-positive immunoreactive material (IBA1+IRM) in the corpus callosum; post-hoc analyses identified a significant increase in GD19 offspring, but not GD10. No change in was observed in the CC. In contrast, maternal immune activation had a significant main effect on GFAP+IRM in the CC at GD19 (not GD10); post-hoc analyses only identified a strong trend towards increased GFAP+IRM in the GD19 offspring, with no white matter changes. This suggests late gestation maternal immune activation causes subtle alterations to microglia and astrocytes in the adult offspring.]]> Tue 04 Jun 2019 13:36:19 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:50563 Tue 01 Aug 2023 10:18:31 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:35487 Thu 30 Jan 2020 13:53:08 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:46136 Thu 29 Jun 2023 12:51:13 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:40979 Thu 21 Jul 2022 09:06:15 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:44697 Thu 20 Oct 2022 09:51:36 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:52648 Thu 19 Oct 2023 15:18:50 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:49926 Thu 15 Jun 2023 11:48:35 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:37534 Thu 04 Feb 2021 16:22:20 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:47846 Thu 02 Feb 2023 15:57:49 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:8145 Sat 24 Mar 2018 08:36:07 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:7899 Sat 24 Mar 2018 08:35:11 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:7273 Sat 24 Mar 2018 08:33:51 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:1924 Sat 24 Mar 2018 08:33:09 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:1358 Sat 24 Mar 2018 08:32:41 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:1343 Sat 24 Mar 2018 08:32:36 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:1537 Sat 24 Mar 2018 08:30:50 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:1173 Sat 24 Mar 2018 08:28:27 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:9880 Sat 24 Mar 2018 08:12:48 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:19902 Sat 24 Mar 2018 08:03:48 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:21485 Salmonella enteritidis) or saline on postnatal days (PNDs) 3 and 5 and later subjected to the formalin test at PNDs 7, 13, and 22. One hour after formalin injection, blood was collected to assess corticosterone responses. Transverse spinal cord slices were also prepared for whole-cell patch clamp recording from lumbar superficial dorsal horn neurons (SDH). Brains were obtained at PND 22 and the hypothalamus was isolated to measure glucocorticoid (GR) and mineralocorticoid receptor (MR) transcript expression using qRT-PCR. Behavioural analyses indicate that at PND 7, no significant differences were observed between saline- or LPS-challenged rats. At PND 13, LPS-challenged rats exhibited enhanced licking (p < .01), and at PND 22, increased flinching in response to formalin injection (p < .05). LPS-challenged rats also displayed increased plasma corticosterone at PND 7 and PND 22 (p < .001) but not at PND 13 following formalin administration. Furthermore, at PND 22 neonatal LPS exposure induced decreased levels of GR mRNA and increased levels of MR mRNA in the hypothalamus. The intrinsic properties of SDH neurons were similar at PND 7 and PND 13. However, at PND 22, ipsilateral SDH neurons in LPS-challenged rats had a lower input resistance compared to their saline-challenged counterparts (p < .05). These data suggest neonatal LPS exposure produces developmentally regulated changes in formalin-induced behavioural responses, corticosterone levels, and dorsal horn neuron properties following noxious stimulation later in life. These findings highlight the importance of immune activation during the neonatal period in shaping pain sensitivity later in life. This programming involves both spinal cord neurons and the HPA axis.]]> Sat 24 Mar 2018 08:03:35 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:20532 Sat 24 Mar 2018 08:02:41 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:19648 Sat 24 Mar 2018 08:01:08 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:21584 Sat 24 Mar 2018 08:00:44 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:17701 Sat 24 Mar 2018 07:57:31 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:5597 Sat 24 Mar 2018 07:49:21 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:5524 Sat 24 Mar 2018 07:46:40 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:27703 Sat 24 Mar 2018 07:40:10 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:26149 +) and somatostatin (SST⁺) IWMNs was determined in the white matter of the corpus callosum in two rostrocaudally adjacent areas in the 12week old offspring of GD10 (n=10) or GD19 polyI:C dams (n=18) compared to controls (n=20). NeuN⁺ IWMN density trended to be higher in offspring from dams exposed to polyI:C at GD19, but not GD10. A subpopulation of these NeuN⁺ IWMNs was shown to express SST. PolyI:C treatment of dams induced a significant increase in the density of SST⁺ IWMNs in the offspring when delivered at both gestational stages with more regionally widespread effects observed at GD19. A positive correlation was observed between NeuN⁺ and SST⁺ IWMN density in animals exposed to polyI:C at GD19, but not controls. This is the first study to show that MIA increases IWMN density in adult offspring in a similar manner to that seen in the brain in schizophrenia. This suggests the MIA model will be useful in future studies aimed at probing the relationship between IWMNs and schizophrenia.]]> Sat 24 Mar 2018 07:35:26 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:38431 Mon 29 Jan 2024 18:00:59 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:32572 Mon 23 Sep 2019 12:39:53 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30987 Mon 20 Nov 2023 14:18:12 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:54764 Mon 11 Mar 2024 15:00:48 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:47891 Mon 06 Feb 2023 12:21:53 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:33142 Mon 03 Sep 2018 12:55:09 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:31493 2 = 0.45). Analysis of HRV parameters revealed significant reduction in both RMSSD (p = 0.02, t = 2.62, η2 = 0.43) and SDRR (p = 0.01, t = 2.90, η2 = 0.45) in the forward ride; no such changes were found in the backward ride. We also found that amplitude of phasic changes in forehead skin conductance increased significantly in both ride directions. This increase however was significantly lower (p<0.05) in backward ride when compared to the forward ride. When assessed immediately post-ride, subjects reported significantly lower (p = 0.04) subjective symptom intensity after the reverse ride compared to the forward ride. We conclude that the direction of visual flow has a significant effect on the symptoms reported by the subjects and on the physiological changes during cybersickness.]]> Fri 21 Jun 2019 18:10:05 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:35486 Fri 16 Aug 2019 13:43:22 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:36750 Fri 03 Jul 2020 09:05:42 AEST ]]>